Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Electrochemical evidences in oxidation of acetaminophen in the presence of glutathione and N acetylcysteine Chemical Communications (RSC Publishing) Glutathione Benefits: The Antioxidant You've Never Heard Of Frontiers Paracetamol Induced Glutathione Consumption: Is There a Link With Severe COVID 19 Illness? The Truth About Why Most Glutathione Supplements Don't Work! Synergy Institute Acupuncture & Chiropractic Is Tylenol Safe During Pregnancy? Understanding Risk Factors Seeking Health